![]() Part 2 Design of scaffold for surface display # 2.1 Build a more controllable display system # Additionally, Candida tropicalis has a long history of use in the manufacture of long-chain acids, which provides a solid basis for its industrial uses. Consequently, it is crucial to set up the system for Candida tropicalis. The Candida tropicalis surface display technology is not as advanced as other surface display systems. It was discovered that the uracil nutrition-deficient strain could not develop on a solid medium without uracil and that the number of colonies grew as the uracil concentration rose. On this plate are various uracil concentrations for the strains. As a result, these bacteria are now uracil nutrition-deficient. To prevent uracil from leaking into the environment, we employed CRISPR-Cas9 technology to knock out the essential genes for uracil synthesis in wild strains. Good safety #Ĭandida tropicalis is a strain with class one biosafety. HuCAL Fab-F-Spy2-H Negative Control Antibody 1ġ Monovalent Fab antibody with (F) DYKDDDDK-, (Spy2) SpyTag version 2, and (H) His-6-tags.Part 1 Selection of a robust host # 1.1 Introduction of Candida tropicalis # Strong viability #Ĭandide tropicalis can survive in environments with acids and phenolic compounds by absorbing alkanes and fatty acids as carbon sources. Sp圜atcher3 fused to the hinge region, CH2, and CH3 of rabbit IgG ![]() Sp圜atcher3 fused to the hinge region, CH2, and CH3 of mouse IgG2a Sp圜atcher3 fused to the hinge region, CH2, and CH3 of human IgA Sp圜atcher3 fused to the hinge region, CH2, and CH3 of human IgG4-Pro (S228P) Sp圜atcher3 fused to the hinge region, CH2, and CH3 of human IgG4 Sp圜atcher3 fused to the hinge region, CH2, and CH3 of human IgG3 Sp圜atcher3 fused to the hinge region, CH2, and CH3 of human IgG2 Sp圜atcher3 fused to the hinge region, CH2, and CH3 of human IgG1 Sp圜atcher3 with an engineered cysteine residue use for site-specific conjugation to a label of choiceīiSp圜atcher2 with three engineered cysteine residues conjugated to biotinīiSp圜atcher2 with one engineered cysteine residue use for site-specific conjugation to a label of choiceīiSp圜atcher2 with three engineered cysteine residues use for site-specific conjugation to a label of choice Sp圜atcher2 with an engineered cysteine residue use for site-specific chemical conjugation to a label of choice Sp圜atchers can also be purchased from our catalog for coupling by the user to an antibody or protein with a reactive SpyTag. Sp圜atchers can be coupled to recombinant Fab antibodies as part of our TrailBlazer Antibody Custom Service. Furthermore, starting with a prelabeled Catcher enables site-directed conjugation and results in improved antibody performance.Īll Sp圜atchers and their derivatives (Table 1) are compatible with our recombinant antibodies that incorporate a SpyTag2 at the C-terminus of the heavy chain (format Fab-F-Spy2-H). ![]() We have added SpyTag to our recombinant Fab antibodies and produced a set of Sp圜atchers to 'glue' to the Fab to make TrailBlazer Antibodies.Ĭoupling an antibody with a SpyTag to different Catcher proteins increases assay design possibilities by allowing the rapid assembly of multiple, stable products, including bivalent Fabs, and Ig-like antibodies with different isotypes. The innovative SpyTag and Sp圜atcher technology, also termed 'molecular superglue', has been employed by the scientific community in many ways to build proteins with novel properties, and address the challenges of detecting and controlling proteins. ![]() Build Better Antibodies Using Molecular Superglue ![]()
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